The effect of dental gel formulation on human primary fibroblasts - an in vitro study.
Identifieur interne : 000023 ( Main/Exploration ); précédent : 000022; suivant : 000024The effect of dental gel formulation on human primary fibroblasts - an in vitro study.
Auteurs : Barbara Sterczala [Pologne] ; Julita Kulbacka [Pologne] ; Jolanta Saczko [Pologne] ; Marzena Dominiak [Pologne]Source :
- Folia histochemica et cytobiologica [ 1897-5631 ] ; 2020.
Descripteurs français
- KwdFr :
- Adhésifs tissulaires (toxicité), Collagène de type III (métabolisme), Fibroblastes (effets des médicaments et des substances chimiques), Gels (toxicité), Gencive (cytologie), Humains (MeSH), Mouvement cellulaire (effets des médicaments et des substances chimiques), Prolifération cellulaire (effets des médicaments et des substances chimiques), Survie cellulaire (effets des médicaments et des substances chimiques).
- MESH :
- cytologie : Gencive.
- effets des médicaments et des substances chimiques : Fibroblastes, Mouvement cellulaire, Prolifération cellulaire, Survie cellulaire.
- métabolisme : Collagène de type III.
- toxicité : Adhésifs tissulaires, Gels.
- Humains.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Collagen Type III.
- cytology : Gingiva.
- drug effects : Cell Movement, Cell Proliferation, Cell Survival, Fibroblasts.
- chemical , toxicity : Gels, Tissue Adhesives.
- Humans.
Abstract
INTRODUCTION
In ordinary dental practice, the dentist often meets with patients suffering from ulcers, aphtha with edema, bleeding gums, bothersome burning, and dry mouth. These are prosthetic, orthodontic patients, after surgery, in various age ranges. Protefix® gel is a soothing and regenerating preparation aimed at patients with mucosal problems. The aim of our study was to evaluate the protective properties and the safety of Protefix® gel application after dental procedures in vitro.
MATERIAL AND METHODS
Human gingival fibroblasts (HGFs) were isolated from normal gingival tissues, cultured to full monolayer and exposed to Protefix® gel in the concentration from 1 to 100%. The viability of cells was examined by MTT assays. Cell migration as a response of treated cells was assessed. The expression of collagen III was estimated by immunocytochemistry after 20 minutes or 24 hours incubation with Protefix® gel.
RESULTS
The obtained results indicated that the verified gel significantly stimulated fibroblasts' proliferation, and mitochondrial activity determined by MTT assay increased almost two-fold for lower gel concentrations. The immunohistochemical detection of collagen III revealed an increased expression after incubation with 5% gel.
CONCLUSIONS
The results proved that the gel is safe for cells derived from human gingiva and moreover has regenerative properties, which can be of great importance in the treatment of gingivitis after retraction and surgical procedures, or even ordinary daily injuries of oral cavity.
DOI: 10.5603/FHC.a2020.0013
PubMed: 32567043
Affiliations:
Links toward previous steps (curation, corpus...)
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an in vitro study.</title><author><name sortKey="Sterczala, Barbara" sort="Sterczala, Barbara" uniqKey="Sterczala B" first="Barbara" last="Sterczala">Barbara Sterczala</name><affiliation wicri:level="1"><nlm:affiliation>Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland. barbara.sterczala@op.pl.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Dental Surgery, Wroclaw Medical University, Wroclaw</wicri:regionArea><wicri:noRegion>Wroclaw</wicri:noRegion></affiliation></author><author><name sortKey="Kulbacka, Julita" sort="Kulbacka, Julita" uniqKey="Kulbacka J" first="Julita" last="Kulbacka">Julita Kulbacka</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular and Cell Biology, Wroclaw Medical University, Wroclaw, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Molecular and Cell Biology, Wroclaw Medical University, Wroclaw</wicri:regionArea><wicri:noRegion>Wroclaw</wicri:noRegion></affiliation></author><author><name sortKey="Saczko, Jolanta" sort="Saczko, Jolanta" uniqKey="Saczko J" first="Jolanta" last="Saczko">Jolanta Saczko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular and Cell Biology, Wroclaw Medical University, Wroclaw, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Molecular and Cell Biology, Wroclaw Medical University, Wroclaw</wicri:regionArea><wicri:noRegion>Wroclaw</wicri:noRegion></affiliation></author><author><name sortKey="Dominiak, Marzena" sort="Dominiak, Marzena" uniqKey="Dominiak M" first="Marzena" last="Dominiak">Marzena Dominiak</name><affiliation wicri:level="1"><nlm:affiliation>Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Dental Surgery, Wroclaw Medical University, Wroclaw</wicri:regionArea><wicri:noRegion>Wroclaw</wicri:noRegion></affiliation></author></analytic><series><title level="j">Folia histochemica et cytobiologica</title><idno type="eISSN">1897-5631</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Movement (drug effects)</term><term>Cell Proliferation (drug effects)</term><term>Cell Survival (drug effects)</term><term>Collagen Type III (metabolism)</term><term>Fibroblasts (drug effects)</term><term>Gels (toxicity)</term><term>Gingiva (cytology)</term><term>Humans (MeSH)</term><term>Tissue Adhesives (toxicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adhésifs tissulaires (toxicité)</term><term>Collagène de type III (métabolisme)</term><term>Fibroblastes (effets des médicaments et des substances chimiques)</term><term>Gels (toxicité)</term><term>Gencive (cytologie)</term><term>Humains (MeSH)</term><term>Mouvement cellulaire (effets des médicaments et des substances chimiques)</term><term>Prolifération cellulaire (effets des médicaments et des substances chimiques)</term><term>Survie cellulaire (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Collagen Type III</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Gencive</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Gingiva</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Movement</term><term>Cell Proliferation</term><term>Cell Survival</term><term>Fibroblasts</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Fibroblastes</term><term>Mouvement cellulaire</term><term>Prolifération cellulaire</term><term>Survie cellulaire</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Collagène de type III</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Gels</term><term>Tissue Adhesives</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Adhésifs tissulaires</term><term>Gels</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>INTRODUCTION</b></p><p>In ordinary dental practice, the dentist often meets with patients suffering from ulcers, aphtha with edema, bleeding gums, bothersome burning, and dry mouth. These are prosthetic, orthodontic patients, after surgery, in various age ranges. Protefix® gel is a soothing and regenerating preparation aimed at patients with mucosal problems. The aim of our study was to evaluate the protective properties and the safety of Protefix® gel application after dental procedures in vitro.</p></div><div type="abstract" xml:lang="en"><p><b>MATERIAL AND METHODS</b></p><p>Human gingival fibroblasts (HGFs) were isolated from normal gingival tissues, cultured to full monolayer and exposed to Protefix® gel in the concentration from 1 to 100%. The viability of cells was examined by MTT assays. Cell migration as a response of treated cells was assessed. The expression of collagen III was estimated by immunocytochemistry after 20 minutes or 24 hours incubation with Protefix® gel.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The obtained results indicated that the verified gel significantly stimulated fibroblasts' proliferation, and mitochondrial activity determined by MTT assay increased almost two-fold for lower gel concentrations. The immunohistochemical detection of collagen III revealed an increased expression after incubation with 5% gel.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The results proved that the gel is safe for cells derived from human gingiva and moreover has regenerative properties, which can be of great importance in the treatment of gingivitis after retraction and surgical procedures, or even ordinary daily injuries of oral cavity.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32567043</PMID><DateCompleted><Year>2021</Year><Month>05</Month><Day>03</Day></DateCompleted><DateRevised><Year>2021</Year><Month>05</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1897-5631</ISSN><JournalIssue CitedMedium="Internet"><Volume>58</Volume><Issue>2</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Folia histochemica et cytobiologica</Title><ISOAbbreviation>Folia Histochem Cytobiol</ISOAbbreviation></Journal><ArticleTitle>The effect of dental gel formulation on human primary fibroblasts - an in vitro study.</ArticleTitle><Pagination><MedlinePgn>156-161</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5603/FHC.a2020.0013</ELocationID><Abstract><AbstractText Label="INTRODUCTION" NlmCategory="BACKGROUND">In ordinary dental practice, the dentist often meets with patients suffering from ulcers, aphtha with edema, bleeding gums, bothersome burning, and dry mouth. These are prosthetic, orthodontic patients, after surgery, in various age ranges. Protefix® gel is a soothing and regenerating preparation aimed at patients with mucosal problems. The aim of our study was to evaluate the protective properties and the safety of Protefix® gel application after dental procedures in vitro.</AbstractText><AbstractText Label="MATERIAL AND METHODS" NlmCategory="METHODS">Human gingival fibroblasts (HGFs) were isolated from normal gingival tissues, cultured to full monolayer and exposed to Protefix® gel in the concentration from 1 to 100%. The viability of cells was examined by MTT assays. Cell migration as a response of treated cells was assessed. The expression of collagen III was estimated by immunocytochemistry after 20 minutes or 24 hours incubation with Protefix® gel.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The obtained results indicated that the verified gel significantly stimulated fibroblasts' proliferation, and mitochondrial activity determined by MTT assay increased almost two-fold for lower gel concentrations. The immunohistochemical detection of collagen III revealed an increased expression after incubation with 5% gel.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The results proved that the gel is safe for cells derived from human gingiva and moreover has regenerative properties, which can be of great importance in the treatment of gingivitis after retraction and surgical procedures, or even ordinary daily injuries of oral cavity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sterczala</LastName><ForeName>Barbara</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland. barbara.sterczala@op.pl.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kulbacka</LastName><ForeName>Julita</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Molecular and Cell Biology, Wroclaw Medical University, Wroclaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saczko</LastName><ForeName>Jolanta</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Molecular and Cell Biology, Wroclaw Medical University, Wroclaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dominiak</LastName><ForeName>Marzena</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Poland</Country><MedlineTA>Folia Histochem 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UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024061" MajorTopicYN="N">Collagen Type III</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005347" MajorTopicYN="N">Fibroblasts</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005782" MajorTopicYN="N">Gels</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005881" MajorTopicYN="N">Gingiva</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014014" MajorTopicYN="N">Tissue Adhesives</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Protefix® gel</Keyword><Keyword MajorTopicYN="N">cell proliferation</Keyword><Keyword MajorTopicYN="N">collagen III</Keyword><Keyword MajorTopicYN="N">human gingival fibroblasts</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2021</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32567043</ArticleId><ArticleId IdType="pii">VM/OJS/J/63479</ArticleId><ArticleId IdType="doi">10.5603/FHC.a2020.0013</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Sterczala, Barbara" sort="Sterczala, Barbara" uniqKey="Sterczala B" first="Barbara" last="Sterczala">Barbara Sterczala</name></noRegion><name sortKey="Dominiak, Marzena" sort="Dominiak, Marzena" uniqKey="Dominiak M" first="Marzena" last="Dominiak">Marzena Dominiak</name><name sortKey="Kulbacka, Julita" sort="Kulbacka, Julita" uniqKey="Kulbacka J" first="Julita" last="Kulbacka">Julita Kulbacka</name><name sortKey="Saczko, Jolanta" sort="Saczko, Jolanta" uniqKey="Saczko J" first="Jolanta" 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